All result files generated by NoiSee will be placed in a new sub-folder located in the same directory as the input image.If unsure, check our provided beads sample data to see how to adjust your acquisition accordingly. Data is expected to be 2D+t, with the focus (Z-plane) set to the center of the beads.The input file format has to be readable by Bio-Formats.In the image field specify the path to a time lapse beads image.Otherwise, you can also locate the NoiSee macros in the Plugins › NoiSee menu.įirst, the parameters dialog will appear with the following input fields / options: Type “ NoiSee” in the quick search bar, select “ Bead Analysis” and click “ Run”. “ laser off”) image, both single frame (no Z-stack, no time-lapse). The Fluorescein Method for an acquisition of a fluorescent solution and a dark field (i.e.NOTE: this method will NOT work for sub-resolution beads! The beads should have a diameter of at least ten pixels in the acquired image. The Beads Method for a time-lapse acquisition of fluorescent beads.NoiSee consists of two independent macros, one for each type of analysis: InstallationĮnable the NoiSee update site and restart Fiji.
NoiSee is an easy-to-use ImageJ macro suite for measuring SNR (signal-to-noise ratio) of confocal microscopes. If you’d like to help, check out the how to help guide! The content of this page has not been vetted since shifting away from MediaWiki.
